FACTORS INFLUENCING THE AUTOLOGOUS MIXED LYMPHOCYTE-REACTION IN RATS - EFFECT OF XENOGENEIC SERUM-PROTEIN

  • 1 January 1981
    • journal article
    • research article
    • Vol. 44  (3) , 499-507
Abstract
Mixed culture of nylon non-adherent (T-enriched) spleen cells or lymph node cells of ACI/N or F344 rats with mitomycin C-treated autologous spleen cells resulted in the increased DNA synthesis of the responder T cells when they were cultured in medium containing fetal calf serum (FCS) or bovine serum albumin. This autologous mixed lymphocyte reaction (AMLR) proceeded little when the cells were cultured in medium containing autologous or allogeneic rat serum, irrespective of the responder:stimulator cell ratio and to the culture period. The AMLR response in FCS was greatly reduced if a relatively large number of macrophages were present in the responder cell population. The AMLR response is probably unlikely to occur in a physiological condition or in vivo.