Relation of Oxygen and Yolk to Retinal Pigmentation in Chick Embryos Subjected to Yolk-Sac Perfusion.

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Abstract
Summary Constant perfusion of a salts-glucose solution through the yolk sacs of 3-or 4-day chick embryos resulted in poor survival and abnormally pale eyes. Incubation in a 40% oxygen atmosphere in the presence of a yolk-supplemented medium permitted virtually normal pigmentation, and greatly enhanced survival. It is concluded that both yolk and oxygen must be present for normal development of retinal pigmentation.

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