Ribonucleic Acid of Foot-and-Mouth Disease Virus: An Ultrasensitive Plaque Assay.
- 1 December 1966
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 123 (3) , 939-945
- https://doi.org/10.3181/00379727-123-31644
Abstract
Summary Foot-and-mouth disease virus RNA infectivity for calf-kidney cultures was 102- to 107-fold higher in the presence of DEAE-dextran than in its absence. By comparison, this polycation had no effect on the sensitivity of the plaque assay of the intact virus. At an optimum concentration of DEAE-dextran of about 1000 μg/ml, the infectivity of different lots of RNA ranged from 10-2 to 102 times that of the virus. A linear relationship between plaque numbers and dilution was observed, and the assay was highly reproducible. DEAE-dextran did not raise the temperature of half-melting of the RNA.This publication has 1 reference indexed in Scilit:
- Influence of Acid Polysaccharides on Plaque Formation by Influenza A2 and B VirusesExperimental Biology and Medicine, 1963