The livers with the gall bladders in situ were carefully removed from 43 human cadavers at autopsy and the surface of the liver and gall bladder were thoroughly washed with Fe-free physiologic NaCl soln. A platinum-iridium needle was then inserted into the gall bladder, and the bile dripping through it was collected in an Fe-free glass flask. Fe was (letd. according to the method of Lintzel (Zeitschr. Exper. Med. 86 269. 1933). All reagents and apparatus used were Fe-free. To 0.5 ml. of the bile in a micro Kjeldahl flask were added 20 drops conc. H2SO4 and 1 ml. HN03, and the mixture was ashed over a small flame. More HNO3, sulfurous acid and H2O2 were added to complete the ashing. The pH of the soln. was adjusted to 5-6 with conc. NH40H and N sulfurous acid and the soln. was poured into a 10 ml. cylinder. To the cylinder were added 0.5 ml. 2% hydro-quinone in N/20 H2SO4 soln. and 0.5 ml. 0.2%,[alpha],[alpha]-dipyridyl in N/20 H2SO4 and the cylinder was filled to the mark with water. The color developed was measured in the step-photometer and the amt. of Fe present then calculated. One ml. of the bile was dried to constant weight in the drying oven to obtain the amt. of dry substance present. The samples of bile contained 0.15-1.99 rag. Fe/100 ml., and there did not seem to be any correlation between the amt. of Fe in the bile and the kind of disease from which the patient died. It was estimated that 1-3 mg. of Fe is excreted by way of the bile into the intestinal tract daily. The amt. of Fe in the bile samples decreased as the amt. of dry substance obtained from the respective bile samples increased. It was suggested that the Fe was reabsorbed in the gall bladder and bile ducts as the bile became concd, and that the Fe was probably in the easily absorbable ferrous form. Since human subjects which receive an Fe-deficient diet do not excrete Fe in the feces, the Fe excreted in the bile into the intestinal tract must be reabsorbed. A human subject received a diet of 300 g. rice, 200 ml. goat''s milk, 60 g. butter fat and 100 g. cane sugar, which afforded him 3.2 mg. Fe/day, for 5 days. His Fe excretion in the feces decreased to 3 mg. per day. On the 6th and 7th days he received a supplement of 0.15 g. [alpha],[alpha]-dipyridyl/day, and his fecal Fe excretion increased to 4.9 mg. Fe/day. A 2d expt. with a diet which afforded only 0.37 mg. Fe/day gave similar results. It was concluded that the [alpha],[alpha]-dipyridyl formed an unresorbable complex with the Fe excreted in the bile, and this Fe was then recovered in the feces. The meconium in the intestine of newborn infants contained 1.5-3 mg. Fe, which was believed to be due to the bile excreted into the intestine during fetal life.