PASSIVE HEMAGGLUTINATION REACTION USING FORMALINIZED SHEEP ERYTHROCYTES TREATED WITH TANNIC-ACID AND COATED WITH EXOTOXIN OF PSEUDOMONAS-AERUGINOSA

  • 1 January 1978
    • journal article
    • research article
    • Vol. 48  (6) , 497-501
Abstract
Passive hemagglutination (PHA) tests using fixed sheep erythrocytes coated with exotoxin of P. aeruginosa were devised for estimating antibodies of the exotoxin. No serological cross-reactivity among exotoxin OEP, protease and elastase of P. aeruginosa was found by the PHA tests. The exotoxin PHA reaction was more sensitive than the neutralizing reaction of skin necrotizing activity of exotoxin by guinea pigs. Of 40 sera of patients with P. aeruginosa infections, 13 showed PHA titers from 40-1280, while those of 20 healthy human sera were under 40.