INTERLEUKIN-1 ACCELERATES AUTOCRINE GROWTH OF MYELOMA CELLS THROUGH INTERLEUKIN-6 IN HUMAN MYELOMA

  • 1 June 1989
    • journal article
    • research article
    • Vol. 73  (8) , 2145-2148
Abstract
Recombinant interleukin 1.alpha. (rIL-1.alpha.) augmented proliferation of freshly isolated myeloma cells as well as B-cell stimulatory factor 2 (BSF-2)/interleukin-6 (IL-6). Recombinant IL-1.alpha.-induced proliferation was partially inhibited by anti-IL-6 antibody. In the culture supernatants of rIL-1.alpha.-stimulated myeloma cells IL-6 activities, which were measured by using an IL-6-dependent murine hybridoma clone, MH60.BSF2, were increased, when compared with those in the culture supernatants of nonstimulated myeloma cells. Furthermore, IL-6 messenger RNA (mRNA) expression was also augmented in IL-1.alpha.-stimulated myeloma cells. Therefore rIL-1.alpha. stimulates myeloma cells to produce IL-6, which consequently augments proliferation of myeloma cells. Thus, IL-1 can accelerate autocrine growth of myeloma cells through IL-6.

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