A new, highly sensitive method for the detection and quantification of penicillin-binding proteins
- 1 April 1993
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 291 (1) , 19-21
- https://doi.org/10.1042/bj2910019
Abstract
A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.Keywords
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