Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin
- 9 August 1990
- journal article
- Published by Springer Nature in Nature
- Vol. 346 (6284) , 534-539
- https://doi.org/10.1038/346534a0
Abstract
Enzymatic synthesis of DNA from the simian virus 40 origin of DNA replication has been reconstituted in vitro with eight purified components. DNA polymerase alpha-primase complex first initiates DNA synthesis at the replication origin and continues as the lagging strand polymerase. Subsequently, the DNA polymerase delta complex initiates replication on the leading strand template. Some prokaryotic DNA polymerase complexes can replace the eukaryotic polymerase delta complex. A model for polymerase switching during initiation of DNA replication is presented.Keywords
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