Dependence of cell yield and specific growth rate ofCandida boidinii on temperature during continuous cultivation

Abstract
Investigation of the dependence of cell yield ofCandida boidinii in a chemostat and specific growth rate in a turbidostat on temperature was carried out using methanol as substrate. Each of the curves obtained had only one maximum, the optimum temperature for yield (Y s )and for specific growth rate (μ) being somewhat different. With deviation of temperature from the corresponding optimum the μ value decreases more sharply than that ofY s . The shape of two-factor dependencesY s (μ, T) and μ(S, T) is analyzed on the basis of these data and the known dependences ofY s on μ and μ on the limiting substrate concentrationS. It is shown that the decelerating action of methanol on the growth rate increases at elevated and lower temperatures.

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