DNA microarrays and beyond: completing the journey from tissue to cell
- 1 August 2001
- journal article
- review article
- Published by Springer Nature in Nature Cell Biology
- Vol. 3 (8) , E175-E178
- https://doi.org/10.1038/35087108
Abstract
For the cell biologist, identifying changes in gene expression using DNA microarrays is just the start of a long journey from tissue to cell. We discuss how chip users can first filter noise (false-positives) from daunting microarray datasets. Combining laser capture microdissection with real-time polymerase chain reaction and reverse transcription is a helpful follow-up step that allows expression of selected genes to be quantified in populations of recovered cells. The voyage from chip to single cell can be completed using sensitive new in situ hybridization and immunohistochemical methods based on tyramide signal amplification.Keywords
This publication has 24 references indexed in Scilit:
- A new approach for filtering noise from high-density oligonucleotide microarray datasetsNucleic Acids Research, 2001
- Genes Expressed in Human Tumor EndotheliumScience, 2000
- Functional Discovery via a Compendium of Expression ProfilesCell, 2000
- Sensitive Immunoassay of Tissue Cell Proteins Procured by Laser Capture MicrodissectionThe American Journal of Pathology, 2000
- Computational methods for theidentification of differential and coordinated gene expressionHuman Molecular Genetics, 1999
- Gene expression informatics —it's all in your mineNature Genetics, 1999
- High density synthetic oligonucleotide arraysNature Genetics, 1999
- Fluorochrome-labeled Tyramides: Use in Immunocytochemistry and Fluorescence In Situ HybridizationJournal of Histochemistry & Cytochemistry, 1997
- Laser Capture MicrodissectionScience, 1996
- Real time quantitative PCR.Genome Research, 1996