Release of cartilage proteoglycan degrading enzyme activity by thioglycollate stimulated mouse peritoneal macrophages in culture
- 1 December 1979
- journal article
- research article
- Published by Springer Nature in Inflammation Research
- Vol. 9 (5-6) , 488-496
- https://doi.org/10.1007/bf01968117
Abstract
Media from cultured mouse peritoneal macrophages were tested for cartilage proteoglycan degrading activity using S35-labelled rabbit ear cartilage. Media samples collected at 2-day intervals contained increasing amounts of activity between days two and six. This activity was activated by trypsin and antagonized by chelating agents. The macrophage products induced release of the proteoglycan component of cartilage as determined by biochemical and histological methods without affecting the collagen component. Media from cells incubated with hydrocortisone were devoid of proteoglycan degrading activity.This publication has 49 references indexed in Scilit:
- Prostaglandin and thromboxane synthesis in a pure macrophage population and the inhibition, by E‐type prostaglandins, of chemiluminescenceFEBS Letters, 1978
- Cellular control of collagen breakdown in rheumatoid arthritisInflammation Research, 1978
- Endogenous Activation of Latent Collagenase by Rheumatoid Synovial CellsNew England Journal of Medicine, 1977
- Activation of alveolar macrophage collagenase by a neutral protease secreted by the same cellNature, 1976
- Collagenase inhibition by cationic proteins derived from cartilage and aortaBiochemical and Biophysical Research Communications, 1976
- Macrophage plasminogen activator: Modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors, and cyclic nucleotidesCell, 1976
- Collagenase from rabbit pulmonary alveolar macrophagesBiochemical and Biophysical Research Communications, 1976
- Corticosteroids inhibit prostaglandin production by rheumatoid synoviaNature, 1975
- The synthesis and analytical use of a highly sensitive and convenient substrate of elastaseBiochemical Medicine, 1974
- Breakdown of noncollagenous chondromucoprotein matrix by leukocyte lysosome granule lysates from guinea pig, rabbit, and humanClinical Immunology and Immunopathology, 1973