A High-Yield, High-Purity Elutriation Method for Preparing Human Granulocytes Demonstrating Enhanced Experimental Lifetimes

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Abstract
A counterflow centrifugal elutriation procedure for preparing human granulocytes is described. This method permits isolation of 1-2 × 109 granulocytes with a yield of 70% ± 7% from a unit of whole blood in 3 h. These cells are 99% ±1% pure and 95-99% viable. When compared to cells prepared by a conventional procedure employing hypotonic lysis to remove red cells, the elutriated cells show enhanced oxidative responses resulting from enhanced longevity. Other responses including receptor-mediated uptake of chemoattractant were not significantly different from those of conventionally prepared cells after incubation at 37° prior to stimulation. The population of elutriated granulocytes, however, was significantly more homogeneous and pure than cells prepared conventionally.

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