Hematoxylin Staining of Tissues Embedded in Epoxy Resins
- 1 January 1969
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 44 (1) , 21-25
- https://doi.org/10.3109/10520296909063319
Abstract
Sections of 0.5-2 μ thickness are affixed to slides with albumen adhesive, thoroughly dried, and placed in xylene or toluene for 1 hr, then brought through ethanol to water. Sections of tissue fixed in OsO4 are treated first in 0.1% KMnO4, then with 1.0% oxalic acid, and after rinsing, incubated at 60 C for 12-24 hr in hematoxylin (Harris's or Ehrlich's) and counterstained 10-15 min with 0.5% phloxine B. Permanent preparations are made by clearing and mounting in a synthetic resin. The method requires only easily available reagents and is suitable for routine processing of epoxy sections.Keywords
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