Antigen Levels of Plasminogen Activator Inhibitor Type 1 in Citrated Plasma: Determination of the Contribution of Platelets

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Abstract
To estimate the extent of the release of plasminogen activator inhibitor type 1 (PAI-1) from platelets following their accidental activation in vitro during blood collection, we studied the antigen levels of PAI-1 and β-thromboglobulin (βTG) in plasma and sera from 38 healthy volunteers. For each volunteer, we calculated the ratio of platelet-derived PAI-1/ platelet-derived βTG from the difference in their respective concentrations in serum and plasma. The mean ± SD (0.012 ± 0.003) and median (0.012) ratios were identical before and after 10 or 20 min of venous occlusion. The platelet contribution of PAI-1 in plasma was estimated by multiplying the plasma concentration of βTG (as a marker of platelet activation) with the above-mentioned individual ratio. Under our conditions of blood collection (1/10 vol of 0.1 M citrate, pH 4.5, as anticoagulant, and handling of blood at 4 °C and within 30 min), the platelet contribution to the PAI-1 concentration in plasma was on average 12% and always negligible in plasma with high levels of PAI-1.

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