Regeneration of haploid and dihaploid plants from protoplasts of supersweet (sh2sh2) corn
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 8 (6) , 313-316
- https://doi.org/10.1007/bf00716662
Abstract
Plants were regenerated from maize (Zea mays L.) protoplasts isolated from embryogenic cell suspensions. The donor maize suspension cultures were established from friable callus initiated from microspores of a commercial supersweet hybrid (sh2sh2). The frequency of cell colony formation was higher when protoplasts were cultured on feeder layers of maize cells as compared with a liquid thin layer method. It was demonstrated that haploid and dihaploid soil-grown plants can be regenerated from maize protoplasts isolated from haploid cell cultures.Keywords
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