Kinetics of Bovine Complement

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Abstract
Although bovine serum is non-hemolytic in standard hemolytic complement assays, it reacts with sensitized sheep erythrocytes to form an intermediate which can be lysed with EDTA-treated guinea pig serum. The optimum condition for the formation of EACBo consists of incubating sensitized sheep erythrocytes and bovine serum at 2°C for 10 min in a low ionic strength buffer (glucose-gelatin-barbital-buffered saline r/2 = 0.075) containing calcium 0.00015 M and magnesium 0.0001 M. In constrast to the corresponding human, guinea pig or equine intermediates, the maximum formation of the bovine lytic intermediate is inversely associated with incubation temperature and is enhanced to a greater extent by magnesium ions. The formation of a bovine lytic intermediate with sheep cells sensitized by rabbit antibody suggests that there is no incompatibility of interaction between early-acting bovine complement components and guinea pig terminal complement components or sensitized sheep cells. Thus the inability of bovine serum to lyse sheep erythrocytes probably is a functional difference of the bovine terminal complement components.

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