Relative rates of appearance of nitrogen and sulphur: implications for postprandial synthesis of proteins

Abstract
The purpose of this study was to gain insights on the temporal fate of proteins based on the rate of appearance of waste products of nitrogen (urea) and sulphur (sulphate) metabolism. Urine was collected every 2 h from 25 normal subjects to measure the rates of excretion of urea, creatinine, and sulphate throughout the 24-h cycle. Samples of blood and urine were also obtained over a 4-h period from 10 subjects who consumed a mixed meal containing 0.4 g protein/kg body weight to obtain information on the relative rates of degradation of amino acids with and without sulphur in a noninvasive fashion. The daily excretion (mean ± SEM) of urea, creatinine, and sulphate was 396 ± 28, 14 ± 0.4, and 15 ± 0.6 mmol, respectively; the molar sulphate/nitrogen (S/N) ratio was 2.0 ± 0.1%. There were relatively minor (

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