INVIVO SYNCHRONIZATION OF MURINE SARCOMA 37 ASCITES TUMOR-CELLS BY USE OF HYDROXYUREA

  • 1 January 1976
    • journal article
    • research article
    • Vol. 8  (2) , 151-155
Abstract
Synchrony of DNA synthesis in mouse sarcoma 37 ascites cells was achieved in vivo by injection of 0.75 mg hydroxyurea per gram weight of mouse. DNA synthesis was gauged by comparing 3H-labeled thymidine incorporation, under defined conditions, to total DNA measured colorimetrically. DNA synthesis in the sarcoma 37 cells fell to negligible levels 4 h after injection and remained at or below levels observed in randomly dividing cells up to 18 h after injection. A sharp increase in DNA synthesis occurred at 20 h, followed by a decline again at 22 h after hydroxyurea injection. DNA synthesis in the intestine remained below control values at 20 h.