A Plaque Reduction Neutralization Test for Human Cytomegalovirus.

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Abstract
Summary A plaque technique for human cytomegaloviruses has been developed employing human embryonic lung fibroblast cultures and an overlay containing 2% methyl cellulose. Neutralization tests employing the 80% plaque reduction endpoint were found to give reproducible results. Human adult sera exhibited titers of 1:8 to 1:64. Typing antisera were successfully prepared in monkeys, yielding titers of 1:32 to 1:256. The technical assistance of Mrs. Catherine Green is gratefully acknowledged.