Immunoglobulin light chain determinants on unstimulated and stimulated human blood lymphocytes, assayed by indirect immunofluorescence.

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  • 1 June 1971
    • journal article
    • Vol. 20  (6) , 1099-111
Abstract
The occurrence of immunoglobulin determinants on the surface of lymphocytes from human blood was assessed by indirect immunofluorescent staining of living cells after cultivation with phytohaemagglutinin or other stimulants. While antisera to γ, μ or α-determinants only stained a few cells, antisera to light chain determinants stained a larger proportion of the cells. Positive staining was recognized as `ring' staining comprising smaller or larger parts of the cell surface. The specificity of staining was ascertained by several types of controls.