Deprivation of nerve growth factor rapidly increases purine efflux from cultured sympathetic neurons
- 25 September 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 255 (2) , 315-320
- https://doi.org/10.1016/0014-5793(89)81113-5
Abstract
The efflux of [3H]purines from cultured sympathetic neurons prelabelled with [3H]adenine is accelerated 2–3-fold within hours of nerve growth factor (NGF) withdrawal and is reduced by readdition of NGF. Addition of 8-(4-chlorphenyl-thio) cAMP, which delays neurite degeneration, reduced the enhanced efflux of purines, as did the addition of cycloheximide, MgCl2 and the protease inhibitor tosyl-L-lysine chloromethyl ketone. Colchicine accelerated purine efflux and neurite degeneration but 2-deoxyglucose increased purine efflux without inducing degeneration, suggesting that ATP reduction itself is not the cause of neurite degeneration. The increase in purine efflux is thus an early biochemical event that has diagnostic value for the study of NGF action since deprivation is detected well before irreversible changes become establishedKeywords
This publication has 20 references indexed in Scilit:
- Inhibitors of protein synthesis and RNA synthesis prevent neuronal death caused by nerve growth factor deprivation.The Journal of cell biology, 1988
- cAMP analogs promote survival and neurite outgrowth in cultures of rat sympathetic and sensory neurons independently of nerve growth factor.Proceedings of the National Academy of Sciences, 1988
- Adenosine 5'-triphosphate synthesis and metabolism localized in neurites of cultured sympathetic neuronsNeuroscience, 1987
- Nucleotide specificity for reactivation of organelle movements in permeabilized axonsCell Motility, 1984
- The Biology and Mechanism of Action of Nerve Growth FactorAnnual Review of Biochemistry, 1982
- Organelle movement in axons depends on ATPNature, 1982
- [53] Long-term culture of dissociated sympathetic neuronsPublished by Elsevier ,1979
- Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.Proceedings of the National Academy of Sciences, 1976
- Morphometric analysis of rat superior cervical ganglion after axotomy and nerve growth factor treatmentJournal of Neurocytology, 1976
- RELATION OF ATP AND CREATINE PHOSPHATE TO FAST AXOPLASMIC TRANSPORT IN MAMMALIAN NERVE1Journal of Neurochemistry, 1972