Molecular cloning of the myelin specific enzyme 2′,3′‐cyclic‐nucleotide 3′‐phosphohydrolase
Open Access
- 29 June 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 218 (2) , 261-265
- https://doi.org/10.1016/0014-5793(87)81058-x
Abstract
We describe the isolation of cDNA clones for bovine brain 2′,3′‐cyclic‐nucleotide 3′‐phosphohydrolase (CNPase, EC 3.1.4.37), the third most abundant protein in central nervous system myelin. The cDNA encodes the complete protein (400 amino acids) and hybridizes to a major size species of mRNA in bovine brain tissue, approx. 2.7 kb in size. CNPase mRNA levels do not appear to be affected in quaking dysmyelinating mutant mice. The sequence reveals probable sites for CNPase phosphorylation by cAMP‐dependent protein kinase and a region of homology with haemocyanin.Keywords
This publication has 20 references indexed in Scilit:
- Clathrin light chains contain brain-specific insertion sequences and a region of homology with intermediate filamentsNature, 1987
- Phosphorylation in vivo of the larger subunit of 2′,3′-cyclic nucleotide-3′-phosphohydrolase in rabbit central nervous system myelinBiochemical Society Transactions, 1986
- Synthetic oligonucleotide probes deduced from amino acid sequence dataJournal of Molecular Biology, 1985
- Endogenous cyclic AMP-stimulated phosphorylation of a Wolfgram protein component in rabbit central-nervous-system myelinBiochemical Journal, 1984
- PURIFICATION OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASE FROM BOVINE BRAIN BY IMMUNOAFFINITY CHROMATOGRAPHY: FURTHER BIOCHEMICAL CHARACTERIZATION OF THE PROTEINJournal of Neurochemistry, 1979
- PURIFICATION AND COMPARISON OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASES FROM BOVINE BRAIN AND SPINAL CORDJournal of Neurochemistry, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- A novel manual method for protein-sequence analysisBiochemical Journal, 1976
- 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASE IN THE DEVELOPING CHICK BRAIN AND SPINAL CORDJournal of Neurochemistry, 1968
- ENZYME INACTIVITY OF MYELIN: HISTOCHEMICAL AND BIOCHEMICAL EVIDENCEJournal of Neurochemistry, 1963