Immunological Reactions of Single Pollen Grains, Electrophoresis and Enzymology of Pollen Protein Exudates

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Abstract
Single intact pollen grains of Oenothera organensis, when placed upon a thin layer of agar containing pollen antiserum, produce circular areas of precipitate. Pollen grains from an S2S2 plant do not produce precipitate in S6 antiserum. Pollen grains from an S6S6 plant and an S2′S4′ self-compatible plant produce precipitates in S6 antiserum. Fifty per cent of the pollen grains from an S2S6 plant produce precipitate in S6 antiserum. Protein diffuses into buffer solutions from intact pollen grains within 2⅔12 min. As much as 40 per cent of the total protein diffuses out in an hour. Amylase and invertase were detected in the diffusate from pollen grains. Alkaline and acid phosphatases were confined to the pollen grains and did not diffuse out. The serological precipitates are specific to the incompatibility system.

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