The metabolism of protocatechuic acid by certain micro-organisms. A reassessment of the evidence for the participation of 2:6-dioxa-3:7-dioxobicyclo[3:3:0]octane as an intermediate

Publisher Website
Google Scholar
Abstract
2:6-Dioxa-3:7-dioxobicyclo[3:3:0] octane ("the dilactone") hydrolyses spontaneously and nonenzymically to ([plus or mimus])-muconolactone; this hydrolysis is rapid above pH 7-0. At pH 6.2-6.6, where the hydrolysis rate to low, extracts of Pseudomonas (phthalate-utilizer), P. fluorescens (A3.12) and Nocardia do not metabolize the dilactone at a rate in excess of its rate of hydrolysis. The dilactone is now considered not to be an intermediate in protocatechuate metabolism by these micro-organisms. Two samples of [beta]gamma-dihy-droxyadipic acid failed to act as precursors of [beta]-oxoadipate when used as substrates in the enzyme system which converts protocatechuate into [beta]-oxoadipate.

This publication has 7 references indexed in Scilit: