Isolation of Nucleoli and Localization of Ribonucleic Acid Polymerase I from Soybean Hypocotyl
- 1 December 1975
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 56 (6) , 850-852
- https://doi.org/10.1104/pp.56.6.850
Abstract
An effective method for the isolation of nucleoli from auxin-treated soybean (Glycine max, var. Wayne) hypocotyl was developed by polytron homogenization and sucrose gradient centrifugation. The nucleoli expressed only the alpha-amanitin-insensitive RNA synthetic activity. This activity chromatographed as RNA polymerase I on DEAE-cellulose. It appears that the plant nucleolus, like the animal nucleolus, is the site of localization for RNA polymerase I.Keywords
This publication has 19 references indexed in Scilit:
- Isolation and Partial Characterization of the Multiple Forms of Deoxyribonucleic Acid-dependent Ribonucleic Acid Polymerase in the Mouse Myeloma, MOPC 315Published by Elsevier ,2021
- Isolation and Properties of Nuclei from Control and Auxin-treated Soybean HypocotylPlant Physiology, 1975
- The separation of RNA polymerases I and II achieved by fractionation of plant chromatinBiochemical and Biophysical Research Communications, 1974
- Separation and Partial Characterization of Multiple Ribonucleic Acid Polymerases from Soybean HypocotylPlant Physiology, 1974
- Isolation, purification and characterization of RNA polymerases from wheat germBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- The DNA-directed RNA polymerases of soybeanBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- RNA Polymerase from Eukaryotic CellsEuropean Journal of Biochemistry, 1972
- RNA Polymerase III of Blastocladiella emersonii is MitochondrialNature New Biology, 1971
- Products of RNA Polymerases in HeLa Cell NucleiProceedings of the National Academy of Sciences, 1971
- HISTONE, A SUPPRESSOR OF CHROMOSOMAL RNA SYNTHESISProceedings of the National Academy of Sciences, 1962