Beta-Propiolactone for the Inactivation of Non-A/Non-B Type 1 Hepatitis Virus Capable of Inducing Cytoplasmic Tubular Ultrastructures in Chimpanzees

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Abstract
Non‐A/Non‐B type 1 hepatitis virus may be recognized because it induces characteristic tubular ultrastructures in the hepatocyte cytoplasm of chimpanzees. 3 chimps received 0.1 ml of a chimp serum containing more than 100 chimp infecting units of non‐A/non‐B type 1 hepatitis virus after it had been treated with β‐propiolactone with or without combined ultraviolet irradiation. All of the chimps escaped infection throughout the observation period of 23 weeks. The treatment of the serum with β‐propiolactone at the mildest condition employed (0.05%, 4°C, 20 min) was still effective in inactivating the virus. The susceptibility of the chimps was ascertained by the subsequent challenge with 0.1 ml of the untreated serum which invariably induced non‐A/non‐B type 1 hepatitis in them. On the basis of these results, β‐propiolactone was extremely efficacious for the cold sterilization of non‐A/non‐B type 1 hepatitis virus.