Cells Forming Spleen Colonies At 7 Or 11 Days After Injection Have Different Proliferation Rates

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Abstract
In the early periods (7-9 days) after hemopoietic cell injection, colonies produced by CFU-s [spleen colony forming unit] and by their progeny are identified in the spleen, while at later periods (11 days after injection) only spleen nodules produced by CFU-s persist. The increase in the suicide values of CFU-s after sublethal (2 Gy [grays]) irradiation of mice is associated with a higher proliferation rate of precursors of transitory spleen colonies, but not of CFU-s, as measured by different suicide techniques. During the log-phase of cell growth in a lethally irradiated recipient, the injected CFU-s and CFU-tr [transient spleen colony forming unit] proliferate at a higher rate. Active proliferation of CFU-s and CFU-tr was demonstrated in long-term bone-marrow cultures by the hydroxyurea in-vitro suicide assay. CFU-tr may be the cause of artifactual effects during measurement of hemopoietic stem-cell cycling by CFU-s suicide methods.