Expression of Inflammatory Cytokines (TNF‐αα, IL‐1, IL‐6 and IL‐8) in Colon of Pigs Naturally Infected withSalmonella typhimuriumandS. choleraesuis

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Abstract
Summary: The expression of mRNA encoding tumour necrosis factor‐α(TNF‐α), interleukin‐1 (IL‐1), IL‐6 and IL‐8 was studied, byin situhybridization with a non‐radioactive digoxigenin‐labelled probe, in formalin‐fixed, paraffin wax‐embedded colonic tissue from pigs naturally infected withSalmonella typhimuriumandS. choleraesuis. Byin situhybridization, a distinct positive signal for TNF‐α, IL‐1, IL‐6 and IL‐8 was detected in colon from all 12 infected pigs. Hybridization signals for all four inflammatory cytokines were detected primarily inflammatory cells infiltrating the lamina propria and submucosa. In comparison, expression of all four inflammatory cytokines was minimal in non‐lesional colon of infected pigs and in normal colon from control pigs. The results suggest that these cytokines play an important role in the pathophysiological processes in porcine salmonellosis.