Interference of Niagara Blue 2B with the Teratogenic Action of Trypan Blue.

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Abstract
Single intra-peritoneal injections of a 2% solution of trypan blue into female Sherman rats on the 8th day of gestation produce fetal resorptions and malformations. The numbers of resorptions and malformations are directly related to the amount of trypan blue administered. It is hypothesized that a non-teratogenic substance of similar structure might in some way interfere with the action of trypan blue. The non-teratogenic disazo dye, Niagara blue 2B, was selected for testing because of its close structural similarity to trypan blue and because it apparently has an identical fate following injection. Intraperitoneal injections of Niagara blue 2B (20 mg, 14 mg, or 10 mg of dye per 100 g rat) on the 6th and 7th days of pregnancy were followed by an injection of trypan blue (20 mg, 14 mg, or 10 mg of dye per 100 g rat) on the 8th day. At the highest dose used (20 mg of each dye per 100 g rat) there was no protective or sparing effect. However, at each lower concentration the injection of Niagara blue 2B prior to the injection of trypan blue afforded some measure of protection to the embryo with respect to the number of resorptions and malformations produced. On the other hand, when trypan blue was injected prior to Niagara blue 2B, there was no significant reduction in its teratogenic activity. The results indicate that the non-teratogenic disazo dye, Niagara blue 2B, can afford some measure of protection to the embryo against the lethal and teratogenic action of trypan blue when injected prior to trypan blue in Sherman rats over a selected range in dye concentration. This protection was not complete.