Transcriptional attenuation control of the tylosin‐resistance gene tlrA in Streptomyces fradiae
- 1 November 1994
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 14 (4) , 833-842
- https://doi.org/10.1111/j.1365-2958.1994.tb01319.x
Abstract
The tylosin producer Streptomyces fradiae contains four known resistance genes, two of which (tlrA and tlrD) encode methyltransferases that act on ribosomal RNA at a common site. Expression of tlrA is regulated via transcriptional attenuation. A short transcript, only 411 nucleotides long, terminates 27 nucleotides into the methylase-coding sequence in the uninduced state. Induction of tlrA is proposed to involve a ribosome-mediated conformational change within the mRNA leader that allows transcription to continue beyond the attenuation site, resulting in a transcript about 1450 nucleotides long. Transplantation of tlrD and/or tlrA into Streptomyces albus revealed that the induction specificity of tlrA depends upon the state of the ribosomes and is significantly altered in strains also expressing tlrD.Keywords
This publication has 26 references indexed in Scilit:
- Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora roseaPublished by Elsevier ,2003
- Sequence similarity between macrolide-resistance determinants and ATP-binding transport proteinsGene, 1992
- Cloning and characterization of two genes from Streptomyces lividans that confer inducible resistance to lincomycin and macrolide antibioticsGene, 1991
- Homology between proteins controlling Streptomyces fradiae tylosin resistance and ATP-binding transportGene, 1991
- Cloning of tlrD, a fourth resistance gene, from the tyiosin producer, Streptomyces fradiaeGene, 1991
- ermC leader peptideJournal of Molecular Biology, 1989
- Genetics of Streptomyces Fradiae and Tylosin BiosynthesisAnnual Review of Microbiology, 1988
- Genetics Of Streptomyces Fradiae And Tylosin BiosynthesisAnnual Review of Microbiology, 1988
- The minimal replicon of a Streptomycete plasmid produces an ultrahigh level of plasmid DNAPlasmid, 1986
- Translational Attenuation: The Regulation of Bacterial Resistance to the Macrolide-Lincosamide-Streptogramin B AntibioticCritical Reviews in Biochemistry, 1984