Mechanisms of suppression in Drosophila. IV. Specificity and properties of tyrosyl-tRNA synthetase

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Abstract
Tyrosyl-tRNA synthetase from wild type Drosophila can distinguish tRNATyr of wild type from that of the suppressor mutant su(s)2. The method of enzyme fractionation on DEAE-cellulose can produce 3 different forms of the enzyme. One of these forms is characterized. The apparent Km for K+, Mg2+, ATP, tyrosine and tRNA, as well as its heat stability and molecular weight are given. Spermine does not replace Mg2+ but is inhibitory; a Ki of 1 mM was obtained. Inhibition by p-chloromercuribenzoate results in a Ki of 1 .mu.M. The properties of tyrosyl-tRNA synthetase of Drosophila are compared to the properties of this enzyme from Escherichia coli, Bacillus subtilis and yeast.

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