Primary DNA Damage in Human Blood Lymphocytes ExposedIn Vitroto 2450 MHz Radiofrequency Radiation

Publisher Website
Google Scholar
Abstract
Vijayalaxmi, Leal, B. Z., Szilagyi, M., Prihoda, T. J. and Meltz, M. L. Primary DNA Damage in Human Blood Lymphocytes Exposed In Vitro to 2450 MHz Radiofrequency Radiation. Human peripheral blood samples collected from three healthy human volunteers were exposed in vitro to pulsed-wave 2450 MHz radiofrequency (RF) radiation for 2 h. The RF radiation was generated with a net forward power of 21 W and transmitted from a standard gain rectangular antenna horn in a vertically downward direction. The average power density at the position of the cells in the flask was 5 mW/cm2. The mean specific absorption rate, calculated by finite difference time domain analysis, was 2.135 (±0.005 SE) W/kg. Aliquots of whole blood that were sham-exposed or exposed in vitro to 50 cGy of ionizing radiation from a 137Cs γ-ray source were used as controls. The lymphocytes were examined to determine the extent of primary DNA damage (single-strand breaks and alkali-labile lesions) using the alkaline comet assay with three ...