IMMUNOLOGIC AND BIOLOGIC CHARACTERIZATION OF ANTISERA TO BEEF THYROTROPIN PREPARATIONS*

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Abstract
Antisera to 3 bovine thyrotropin preparations were produced by hyperimmunization of rabbits, and these antisera were immunologically and biologically characterized. Precipitating antibody, apparently specific for thyrotropin, was demonstrated by gel diffusion technics. Parallel immunoelectrophoretic analyses and bioassays for thyroid-stimulating hormone (TSH) activity of segments of agar after electrophoresis confirmed the migration of thyrotropin in an electrical field and support the conclusion that the precipitins are specific for the thyrotropic principle. Luteinizing hormone was found to be immunologically related to, but not identical with thyrotropin. By two different bioassay methods, the anti-TSH antisera were shown to be capable of neutralizing the thyrotropic activity of both homologous and heterologous TSH preparations.