Biochemical Properties of Neoplastic Cell Mitochondria2

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Abstract
Mitochondria from monolayer cultures of Novikoff hepatomacells were studied for their biochemical and structural organization and compared with mitochondria from solid Novikoff hepatoma and normal liver. Cultured cell and tumor mitochondria were similar in both ultrastructure and biochemical properties. Both differed distinctly from liver mitochondria, in that they had drastically reduced activities of several flavoprotein enzymes associated with their outer and Inner membranes and altered morphology of their matrix as evident from electron micrographs. The alterations we observed in the Novikoff hepatoma mitochondria were not the result of varied growth conditions but were inherent in these neoplastic cells. Mitochondria from another neoplastic cell line, hamster melanoma CCL 49, grown in vitro were compared to mitochondria from cultures of a non-neoplastic cell line, hamster embryo Nil 2. Melanoma cell mitochondria showed reductions in outer and inner membrane flavoproteins similar to those observed in Novikoff hepatoma mitochondria. There were significant reductions in the activities of NADH oxidase, NADH-cytochrome c reductase, and adenylate kinase. In addition, monoamine oxidase was absent.

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