Aphidicolin: A specific inhibitor of DNA synthesis in synchronous mastocytoma P-815 cells.
- 1 January 1980
- journal article
- research article
- Published by Elsevier in The Japanese Journal of Pharmacology
- Vol. 30 (3) , 301-308
- https://doi.org/10.1254/jjp.30.301
Abstract
The influence of aphidicolin on cell multiplication and DNA synthesis was examined using synchronous mouse mastocytoma P-815 cells. Aphidicolin was cytotoxic specifically to the cells of the S phase of the cell cycle. This cytotoxicity was reversed by appropriately washing the drug-treated cells, but not by the addition of deoxyribonucleosides. Aphidicolin, a potent inhibitor of DNA synthesis, selectively inhibited the activity of partially purified DNA polymerase .alpha. from the nucleus and the cytosol of mastocytoma cells, but did not affect the activity of DNA polymerase .beta.. Aphidicolin had no effect on the synthesis of RNA and protein, and produced no changes in cell size at least for 1 generation.This publication has 1 reference indexed in Scilit:
- Aphidicolin does inhibit repair replication in Hela cellsBiochemical and Biophysical Research Communications, 1979