Thyroid-Stimulating and Exophthalmos-Producing Activity of Biochemically Altered Thyrotropin1

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Abstract
Biochemical alteration of beef thyrotropin (1–1.5 USP units/mg) was carried out with acetic anhydride to acetylate amino groups, periodate oxidation to destroy any carbohydrate residues with vicinal hydroxyl groups, exposure to neuraminidase to remove sialic acid and exposure to hydrogen chloride-methanol to methylate carboxyl groups. The resulting compounds were compared with the standard preparation of TSH in their ability to increase I131 uptake by the mouse thyroid gland (Querido), to induce exophthalmos in the goldfish and to increase the acid mucopolysaccharide content of the dog thyroid gland (Bollet). Thyroid-stimulating activity was significantly reduced by acetylation and periodate-borohydride treatment, but not by exposure to neuraminidase or methylation. Exophthalmos-producing activity was significantly reduced by acetylation, periodate-borohydride treatment and methylation, but not by exposure to neuraminidase. The activity of the biochemically altered TSH preparations was similar in both thyroid stimulation and exophthalmos production. The production of exophthalmos by standard TSH was reduced when acetylated TSH was given concurrently.

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