PROTEIN SYNTHESIS AND NITROGEN UPTAKE IN SYNCHRONOUSLY DIVIDING CULTURES OFSACCHAROMYCES CEREVISIAE

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Abstract
Protein synthesis in synchronously dividing cultures of Saccharomyces cerevisiae, starting from the appearance of the first generation of buds, continued fairly steadily throughout each of the subsequent division cycles. Although the precise shape of the synthesis curve was not determined, the mean rate of synthesis in the second cycle was approximately double that of the first. In contrast to the continuous rise in protein, the total nitrogen content of the cultures increased by a series of abrupt steps, one in each division cycle, as nitrogen was taken up from the medium. The uptake occurred early in the growth of the cell and was usually completed by the mid-point of the division cycle.

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