Changing patterns of gene expression during sporulation in yeast.

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Abstract
Analysis of RNA isolated from the yeast Saccharomyces cerevisiae reveals a dramatic series of changes in protein coding sequences during sporulation. Shortly after transfer to sporulation medium, mRNA for certain proteins are repressed while a broad array of mRNA for other proteins is induced. Superimposed on this general increase in transcriptional activity is the very strong induction of a particular subset of heat shock mRNA, the same subset that is induced during the normal course of oogenesis in Drosophila. At distinct times later in sporulation, 2 sets of abundant mRNA are coordinately induced. Unlike the earlier changes in the message complement, these changes are unique to sporulating cells. As asci mature, one set of sporulation-specific RNA is selectively degraded. The second set, as well as the broad array of mRNA induced earlier in development, is retained in a highly stable and fully translatable form.