Some properties of 3-hydroxy-3-methylglutaryl-coenzyme A synthase from ox liver
- 1 September 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 173 (3) , 925-928
- https://doi.org/10.1042/bj1730925
Abstract
Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (EC 4.1.3.5) was purified from ox liver, and obtained essentially free from 3-oxoacyl-CoA thiolases. The kinetic behaviour, like that of the synthases from chicken liver and yeast, is compatible with a reaction pathway involving condensation of an acetyl-enzyme with acetoacetyl-CoA. The Km for acetoacetyl-CoA, less than 1 micronM at pH 7.8, may possibly be low enough to permit rapid ketogenesis under physiological conditions without the need for a binary complex between the synthase and oxoacyl-CoA thiolase.This publication has 13 references indexed in Scilit:
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