Comments on the Paper “Relevance of Three-Dimensional Reconstructions of Stain Distributions for Structural Analysis of Biomolecules”
- 31 December 1974
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 356 (2) , 1317-1320
- https://doi.org/10.1515/bchm2.1975.356.2.1317
Abstract
Discrimination of the heavy atom stain against the low contrast image of the light atoms is better in 3 dimensions than in 2 dimensions. In a conventional micrograph, the contrast of light atoms can add up by projection. It is one of the fundamental advantages of 3-dimensional imaging that these ambiguities disappear, because there is the spatial discrimination between the heavy regions and the light regions. The structure of the supporting film, which obscures the object in conventional microscopy will also be discriminated.This publication has 0 references indexed in Scilit: