Properties of Duck Hepatitis Virus

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Abstract
The viability of duck hepatitis virus (DHV) in allantoamniotic (aa) fluids was determined after various holding periods at 25, 37, and 56[degree] C. The ID50 of the virus was determined by titration in 8-day-old embryonated chicken eggs following each treatment period at the three temperature ranges. The ID50 of DHV at 56[degree]C decreased rapidly between 5 and 90 minutes, and at 105 minutes no viable virus could be demonstrated. Exposure of DHV to 37% reduced active virus markedly after 48 hours although some virus was still viable at 7 days but not at 9 days. Virus exposed to 25[degree] C survived with little loss in activity at 11 days. Although virus was still viable at 4 weeks, none was present at 5 weeks. The virus was successfully lyophilized, although the titer of the virus was reduced approximately 2 logs by the process. DHV in aa fluid was exposed to various chemical agents in an effort to obtain a more purified suspension. Titration of the suspensions demonstrated that the virus survived exposure to protamine sulfate, potassium alum, ammonium sulfate, and 30% methyl alcohol for at least 24 hours. DHV remained active after 7 years at -20[degree] C. The IDgg of the virus was not affected when the aa suspensions were adjusted to pH 4.8 and 7.8.

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