In vitro function of granulocyte concentrates following passage through an electromechanical infusion pump

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Abstract
Six units of granulocytes stored for 18 hours at 20 to 24.degree. C without agitation were passed through an electromechanical infusion device pump system (test) (Abbott) and through a 170-.mu.m filter (control). To determine the effect of needle gauge, a 19 or 23 gauge needle was attached to the end of the pump administration set. After passage through the pump, granulocytes were evaluated for signs of cytolysis and functional loss, red cells for hemolysis, platelets for release of .beta.-thromboglobulin, and plasma for C3a and C5a complement activation. There was no evidence (mean .+-. 1 SD) of hemolysis (< 1.0 vs < 1.0 mg/dl), loss of granulocytes (123 .+-. 38 .times. 103/.mu.l vs. 118 .+-. 29 .times. 103/.mu.l), changes in any of several tests of neutrophil function, increased release of .beta.-thromboglobulin (15.8 .+-. 5.8 vs. 17.0 .+-. 6.6%), or C3a complement activation (483 .+-. 221 vs 500 .+-. 200 ng/ml) after passage through the pump system (p > 0.05). No significant differences were seen between the use of a 19 or 23 gauge needle. Based on these in vitro data, we conclude that this pump system is acceptable for use in clinical practice when control over rate and volume of granulocyte administration is important.