Site specificity of DNA methylation and expression of herpes simplex thymidine kinase gene
- 1 July 1986
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 12 (4) , 385-393
- https://doi.org/10.1007/bf01570733
Abstract
Analysis of the methylation pattern of a single-copy herpes simplex virus thymidine kinase gene integrated into the genome of mouse L cells revealed that hypomethylation of five specific Aval sites correlates with expression of the TKgene in all of the cell lines tested. Of these specific sites, one lies 5′ to the coding region, one 3′ to the coding region, and three lie within the coding region of the thymidine kinase gene. Analysis of methylation at a variety of other sites using other methylation-sensitive endonucleases revealed considerable variation in the methylation patterns, apparently unrelated to gene expression and subject to variation with time in culture.This publication has 29 references indexed in Scilit:
- Ultraviolet-induced reactivation, amplification, and hypomethylation of a herpes simplex virus thymidine kinase geneSomatic Cell and Molecular Genetics, 1984
- Insulin-induced reactivation of an inactive herpes simplex thymidine kinase gene.Proceedings of the National Academy of Sciences, 1984
- DNA methylation and the regulation of globin gene expressionCell, 1983
- Expression of transferred thymidine kinase genes is controlled by methylation.Proceedings of the National Academy of Sciences, 1982
- Phenotypic Switching in Cells Transformed with the Herpes Simplex Virus Thymidine Kinase GeneMolecular and Cellular Biology, 1982
- 5-Azacytidine-Induced Reactivation of a Herpes Simplex Thymidine Kinase GeneScience, 1982
- Methylation and gene controlNature, 1982
- DNA Methylation and Gene FunctionScience, 1980
- DNA methylation in the human γδβ-globin locus in erythroid and nonerythroid tissuesCell, 1980
- DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genesNucleic Acids Research, 1979