Application of PCR to Distinguish Common Species of Dermatophytes
- 1 September 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (9) , 3382-3385
- https://doi.org/10.1128/jcm.39.9.3382-3385.2001
Abstract
This report describes the application of PCR fingerprinting for the identification of species and varieties of common dermatophytes and related fungi utilizing as a single primer the simple repetitive oligonucleotide (GACA) 4 . The primer was able to amplify all the strains, producing species-specific profiles for Microsporum canis , Microsporum gypseum , Trichophyton rubrum , Trichophyton ajelloi , and Epidermophyton floccosum. Intraspecific variability was not observed for these species. Instead, three different profiles were observed in the Trichophyton mentagrophytes group.Keywords
This publication has 16 references indexed in Scilit:
- Application of PCR to the identification of dermatophyte fungiJournal of Medical Microbiology, 2000
- Differences among chitin synthase 1 gene sequences inTrichophyton rubrumandT. violaceumMedical Mycology, 2000
- Molecular taxonomy of Trichophyton mentagrophytes and T. tonsuransMedical Mycology, 1999
- Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS regionMedical Mycology, 1999
- Identification of common dermatophytes (Trichophyton,Microsporum,Epidermophyton) using polymerase chain reactionsBritish Journal of Dermatology, 1998
- PCR Identification of Trichophyton Mentagrophytes Var. Interdigitale And T. Mentagrophytes var. Mentagrophytes Dermatophytes with a Random PrimerJournal of Medical Microbiology, 1997
- Identification of pathogenic yeasts of the imperfect genus Candida by polymerase chain reaction fingerprintingElectrophoresis, 1997
- Phytogeny of Epidermophyton floccosum and other dermatophytesMycopathologia, 1996
- Polymerase chain reaction fingerprinting in fungi using single primers specific to minisatellites and simple repetitive DNA sequences: Strain variation in Cryptococcus neoformansElectrophoresis, 1995
- Phylogeny of Nannizzia incurvata, N. gypsea, N. fulva and N. otae by restriction enzyme analysis of mitochondrial DNAMycopathologia, 1990