Systemic enzymatic changes in guinea pigs suffering from experimental allergic encephalomyelitis.

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Abstract
Experimental allergic encephalomyelitis (EAE) is one of the experimental models of human demyelinating diseases and recently also has been regarded as a useful model for studying cell-mediated autoimmune diseases. Because of the possibility of induction of systemic changes in this model, enzymatic changes were investigated in serum and main organs of the diseased animals, including brain, spinal cord, limb muscle, heart muscle, spleen, liver and kidney. The enzymes measured consisted of aspartate aminopeptidase (EC 3.4.11.7), arginine aminopeptidase (EC 3.4.11.6), proline iminopeptidase, leucine aminopeptidase (EC 3.4.11.1), formylmethionine aminopeptidase, phenylalanine aminopeptidase, tripeptide aminopeptidase (EC 3.4.11.4), serine proteinase (EC 3.4.21), dipeptidylaminopeptidase I (EC 3.4.14.1), carboxyl proteinase (EC 3.4.23.5), .alpha.-D-glucosidase (EC 3.2.1.20), .alpha.-D-mannosidase (EC 3.2.1.24), .beta.-N-acetyl-D-glucosaminidase (EC 3.2.1.30), creatine kinase (EC 2.7.3.2), alkaline phosphatase (EC 3.1.3.1), and arylesterase (EC 3.1.1.2). Significant changes of many enzymatic activities occurred in all the organs tested in 1-2 wk after the administration of EAE antigen, myelin basic protein (MBP). Interesting correlations of the pattern of enzymatic changes were seen among most of the organs tested. Those patterns changed in the course of the 2 wk and there remained marked changes characteristic for each organ. This model may represent some type of systemic autoimmune disease.