LytG of Bacillus subtilis Is a Novel Peptidoglycan Hydrolase: The Major Active Glucosaminidase
- 19 December 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 42 (2) , 257-264
- https://doi.org/10.1021/bi020498c
Abstract
LytG (YubE) of Bacillus subtilis is a novel 32 kDa autolysin produced during vegetative growth under the control of EσA RNA polymerase. Muropeptide analysis of vegetative cells of B. subtilis revealed LytG to be the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Overexpression and purification of LytG allowed its biochemical characterization. Despite sequence homology suggesting muramidase activity, LytG is a novel glucosaminidase with exoenzyme activity and may form part of a novel family of autolysins. It is involved in cell division, lysis, and motility on swarm plates.Keywords
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