Genotyping Encephalitozoon hellem Isolates by Analysis of the Polar Tube Protein Gene
Open Access
- 1 June 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (6) , 2191-2196
- https://doi.org/10.1128/jcm.39.6.2191-2196.2001
Abstract
To develop an alternative genotyping tool, the genetic diversity of Encephalitozoon hellem was examined at the polar tube protein (PTP) locus. Nucleotide sequence analysis of the PTP gene divided 24 E. hellem isolates into four genotypes, compared to two genotypes identified by analysis of the internal transcribed spacer of the rRNA gene. The four PTP genotypes differed from each other by the copy number of the 60-bp central repeat as well as by point mutations. A simple PCR test was developed to differentiate E. hellem genotypes based on the difference in the size of PTP PCR products, which should facilitate the genotyping of E. hellem in clinical samples.Keywords
This publication has 51 references indexed in Scilit:
- Close Genotypic Relationship betweenEnterocytozoon bieneusifrom Humans and Pigs and First Detection in CattleJournal of Parasitology, 2000
- Close Genotypic Relationship between Enterocytozoon bieneusi from Humans and Pigs and First Detection in CattleJournal of Parasitology, 2000
- Genetic and phenotypic intraspecific variation in the microsporidian Encephalitozoon hellemPublished by Elsevier ,1999
- Inter- and Intra-species Karyotype Variations Among Microsporidia of the Genus Encephalitozoon as Determined by Pulsed-field Gel ElectrophoresisScandinavian Journal of Infectious Diseases, 1999
- Extrainstestinal Microsporidiosis in AIDS Patients: Clinical Features and Advanced Protocols for Diagnosis and Characterization of the IsolatesThe Journal of Eukaryotic Microbiology, 1997
- Evidence for the existence of genetically distinct strains of Enterocytozoon bieneusiZeitschrift Fur Parasitenkunde-Parasitology Research, 1997
- Two Encephalitozoon cuniculi strains of human origin are infectious to rabbitsParasitology, 1997
- Molecular Epidemiology of Encephalitozoon cuniculi and First Detection of Enterocytozoon bieneusi in Faecal Samples of PigsThe Journal of Eukaryotic Microbiology, 1996
- Isolation and identification of Encephatitozoon hellem from an Italian AIDS patient with disseminated microsporidiosisAPMIS, 1994
- Disseminated Microsporidiosis Due to Encephalitozoon hellem: Pulmonary Colonization, Microhematuria, and Mild Conjunctivitis in a Patient with AIDSClinical Infectious Diseases, 1993