On the barometric method for measurements of ventilation, and its use in small animals

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Abstract
The barometric method is a common technique for measurements of pulmonary ventilation in unrestrained animals. It basically consists of recording the changes in chamber pressure generated during breathing. In fact, as the air inspired is warmed and humidified from the ambient to the pulmonary values, the total pressure in the animal chamber increases; the opposite occurs in expiration. The present commentary is an introduction to this method, briefly reviewing its historical development, the conceptual pitfalls, and potential sources of errors during practical applications.Key words: barometric technique, plethysmography, pulmonary ventilation, respiratory techniques, tidal volume.

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