Sequences of four tRNA genes fromCaenorhabditis elegansand the espression ofC.eleganstRNALeu(anticodon IAG) inXenopusoocytes

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Abstract
Four tRNA genes have been identified in cloned segments of Caenorhabditis elegans DNA by tRNA hybridization and expression after injection into Xenopus laevis oocyte nuclei. From DNA sequencing these are (with DNA anticodon sequences) tRNAAsp (CTC), tRNALeu (AAC), tRNA Lys (CTT) and tRNA Pro(TGG). Their flanking DNA sequences are compared. Two identical tRNA Lys (CTT) genes from different regions of the genome have quite unrelated 5‘ flanking sequences the tRNA synthesized in Xenopus oocytes after injection of the tRNALeu cloned DNA has the modified anticodon JAG. The tRNALeu gene precursor transcript from injected oocytes has short 5‘ and 3‘ additional sequences and Jacks certain of those modified bases found in the processed tRNA.