A Sensitive Radioimmunoassay for Detection of Antibodies to Recombinant Human Interferon-αA

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Abstract
A radioimmunoassay (RIA) for the detection of antibodies to recombinant human leukocyte interferon A (rHuIFN-αA) in human serum has been developed and validated against the standard antiviral neutralization bioassay (ANB). The assay measures the binding of 125I-labeled rHuIFN-αA to immunoglobulins in serum. Aliquots of patients' sera are incubated with 125I-rHuIFN-αA and the complexes formed between antibodies in the sera and the 125I-rHuIFN-αA are precipitated with goat anti-human IgG serum. The radioactivity in the immune precipitate is a measure of the quantity of antibody (if present) in the serum. The sensitivity of this RIA is 5 ng of IgG/ml of serum.